Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007

Ibarra Juárez, Luis Arturo y Eisen, Lars y Bolling, B. G. y Beaty, Barry J. y Blitvich, Bradley J. y Sánchez Casas, Rosa María y Ayala Sulca, Yuri O. y Fernández Salas, Ildefonso (2012) Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007. Medical and veterinary entomology, 26 (1). pp. 351-354. ISSN 0269-283X

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Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007.pdf

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Abstract. In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles, Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reversetranscription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx. quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region.

Tipo de elemento: Article
Palabras claves no controlados: Flavivirus, horse, mosquitoes, seroprevalence, surveillance, West Nile virus.
Materias: Q Ciencia > Q Ciencias en General
Q Ciencia > QH Historia Natural, Biología
Divisiones: Ciencias Biológicas
Usuario depositante: Dra Rosa Maria / RM Sanchez Casas
Creadores:
CreadorEmailORCID
Ibarra Juárez, Luis ArturoNO ESPECIFICADONO ESPECIFICADO
Eisen, LarsNO ESPECIFICADONO ESPECIFICADO
Bolling, B. G.NO ESPECIFICADONO ESPECIFICADO
Beaty, Barry J.NO ESPECIFICADONO ESPECIFICADO
Blitvich, Bradley J.NO ESPECIFICADONO ESPECIFICADO
Sánchez Casas, Rosa MaríaNO ESPECIFICADONO ESPECIFICADO
Ayala Sulca, Yuri O.NO ESPECIFICADONO ESPECIFICADO
Fernández Salas, Ildefonsoifernand1@hotmail.comNO ESPECIFICADO
Fecha del depósito: 31 Jul 2015 22:31
Última modificación: 31 Jul 2015 22:31
URI: http://eprints.uanl.mx/id/eprint/6439

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