Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis

González Ochoa, Guadalupe y Menchaca, Griselda y Hernández, Carlos y Rodríguez, Cristina y Tamez, Reyes y Contreras, Juan (2013) Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis. Viruses, 5 (3). pp. 792-805. ISSN 1999-4915

[img]
Vista previa
Texto
535.pdf - Versión Publicada
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (465kB) | Vista previa
URL o página oficial: http://doi.org/10.3390/v5030792

Resumen

The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 14 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor.

Tipo de elemento: Article
Palabras claves no controlados: rotavirus; severe gastroenteritis; NSP4
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
González Ochoa, GuadalupeNO ESPECIFICADONO ESPECIFICADO
Menchaca, GriseldaNO ESPECIFICADONO ESPECIFICADO
Hernández, CarlosNO ESPECIFICADONO ESPECIFICADO
Rodríguez, CristinaNO ESPECIFICADONO ESPECIFICADO
Tamez, ReyesNO ESPECIFICADONO ESPECIFICADO
Contreras, JuanNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 15 Sep 2020 12:38
Última modificación: 15 Sep 2020 12:38
URI: http://eprints.uanl.mx/id/eprint/14858

Actions (login required)

Ver elemento Ver elemento

Downloads

Downloads per month over past year