Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells

Rendón Huerta, Erika P. y Torres Martínez, Ana y Charles Niño, Claudia y Rivas Estilla, Ana María y Páez, A. y Fortoul, Teresa I. y Montaño, Luis F. (2013) Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells. Annals of hepatology, 12 (4). pp. 448-457. ISSN 1665-2681

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Background. Hepatitis C virus (HCV) infection usually results in long-term viremia. Entry of HCV into the he-patocyte requires claudin-1, -6, -9 and occludin. The efficacy of Pegylated interferon-α (PEG-IFN) treatment against HCV infection increased when ribavirin (RBV) was added to the therapeutic scheme. Our aim was to investigate if PEG-IFN plus RBV regulate claudin expression.Material and methods. HepG2, Huh-7 and Huh-7.5 cells were treated with PEG-IFN-α2a or α2b and/or RBV at different times before obtaining the cytosolic, membrane and cytoskeletal fractions. Claudin-1, 3, 4, 6, and 9, E-cadherin and occludin expression was evaluated by Western blot analysis. Transepithelial electrical resistance (TER) was also determined.Results. Claudin-1, 3, 4, 6, E-cadherin and occludin are constitutively expressed mainly in HepG2 cell membrane. Claudin-1 and E-cadherin cell membrane expression diminished after exposure to PEGIFNα2b (50 ng) + RBV(50 μg); the maximal decrease was observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. The effect was less intense with PEG-IFNα2a. The inhibition of claudin-1 and E-cadherin expression in Huh-7 and Huh-7.5 cells was only observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. TER diminished marginally in the HCV containing hepatoma cells with 200 ng of PEG-IFNα2b + 200 ug of RBV. Claudin-1 mRNA expression level was not affected by the combined treatment.Conclusion. The increased therapeutic efficacy of the PEG-IFNα2b plus RBV treatment could be secondary to the inhibition of claudin-1 and E-cadherin cell membrane expression.

Tipo de elemento: Article
Palabras claves no controlados: Proteínas de unión estrecha, virus de la hepatitis C, Claudins
Materias: R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Divisiones: Medicina
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Rendón Huerta, Erika P.NO ESPECIFICADONO ESPECIFICADO
Torres Martínez, AnaNO ESPECIFICADONO ESPECIFICADO
Charles Niño, ClaudiaNO ESPECIFICADONO ESPECIFICADO
Rivas Estilla, Ana MaríaNO ESPECIFICADONO ESPECIFICADO
Páez, A.NO ESPECIFICADONO ESPECIFICADO
Fortoul, Teresa I.NO ESPECIFICADONO ESPECIFICADO
Montaño, Luis F.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 29 Jun 2022 20:22
Última modificación: 18 Mayo 2023 18:51
URI: http://eprints.uanl.mx/id/eprint/23504

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