Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori

Urrutia Baca, Víctor Hugo y Gomez Flores, Ricardo y De la Garza Ramos, Myriam Angélica y Tamez Guerra, Patricia y Lucio Sauceda, Daniela Guadalupe y Rodríguez Padilla, María Cristina (2019) Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori. Journal of Computational Biology, 26 (10). pp. 1177-1190. ISSN 1557-8666

Vista previa

Texto
29709.pdf - Versión Publicada
Available under License Creative Commons Attribution Non-commercial No Derivatives.
Download (890kB) | Vista previa

Resumen

Helicobacter pylori is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the increase in resistance to antibiotics used in the anti-H. pylori therapy, the development of an effective vaccine is an alternative of great interest, which remains a challenge. Therefore, a rational, strategic, and efficient vaccine design against H. pylori is necessary where the use of the most current bioinformatics tools could help achieve it. In this study, immunoinformatics approach was used to design a novel multi epitope oral vaccine against H. pylori. Our multi epitope vaccine is composed of cholera toxin subunit B (CTB) that is used as a mucosal adjuvant to enhance vaccine immunogenicity for oral immunization. CTB fused to 11 epitopes predicted of pathogenic (UreB170–189, VacA459–478, CagA1103–1122, GGT106–126, NapA30–44, and OipA211–230) and colonization (HpaA33–52, FlaA487–506, FecA437–456, BabA129–149, and SabA540–559) proteins from H. pylori. CKS9 peptide (CKSTHPLSC) targets epithelial microfold cells to enhance vaccine uptake from the gut barrier. All sequences were joined to each other by proper linkers. The vaccine was modeled and validated to achieve a high-quality three-dimensional structure. The vaccine design was evaluated as non allergenic, antigenic, soluble, and with an appropriate molecular weight and isoelectric point. Our results suggest that our newly designed vaccine could serve as a promising anti-H. pylori vaccine candidate.

Tipo de elemento:

Article

Palabras claves no controlados:

Helicobacter pylori, In silico, Multiepitope, Oral vaccine, Reverse vaccinology

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Urrutia Baca, Víctor Hugo	NO ESPECIFICADO	NO ESPECIFICADO
Gomez Flores, Ricardo	rgomez60@hotmail.com	orcid.org/0000-0002-9518-4433
De la Garza Ramos, Myriam Angélica	myriam.garzarm@unal.edu.mx.	NO ESPECIFICADO
Tamez Guerra, Patricia	patamez@hotmail.com	orcid.org/0000-0002-4831-2340
Lucio Sauceda, Daniela Guadalupe	NO ESPECIFICADO	NO ESPECIFICADO
Rodríguez Padilla, María Cristina	NO ESPECIFICADO	NO ESPECIFICADO